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Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile.

Biochemical and biophysical research communications - 25 Dec 2015

Sun Huihui, Wang Hualei, Gao Wenyuan, Chen Lifeng, Wu Kai, Wei Dongzhi

Abstract excerpt

Nitrilase PpL19 from Pseudomonas psychrotolerans L19 can hydrolyze racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess (ee) value of 52.7%. In this study, random mutagenesis combined with site-directed mutagenesis was performed to identify the key residues responsible for nitrilase enantioselectivity. Five enzyme mutants exhibiting distinct selectivity were generated and four "hot spots"...

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PMID: 26577409
DOI: 10.1016/j.bbrc.2015.11.038

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