Conformational conversion may precede or follow aggregate elongation on alternative pathways of amyloid protofibril formation.

Abstract excerpt

A major goal in the study of protein aggregation is to understand how the conformational heterogeneity characteristic of the process leads to structurally distinct amyloid fibrils. The small protein barstar is known to form amyloid protofibrils in multiple steps at low pH: a small oligomer, the A-form, first transforms into a larger spherical higher oligomeric intermediate (HOI), which then self-associates to...

Topics

• Amino Acid Sequence
• Amyloid
• Bacterial Proteins
• Kinetics
• Microscopy, Atomic Force
• Models, Biological
• Molecular Sequence Data
• Mutant Proteins
• Mutation
• Protein Structure, Quaternary
• Spectrometry, Fluorescence