RNA binding by the tat and rev proteins of HIV-1.

doi:10.1016/0300-9084(91)90068-c

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Article

RNA binding by the tat and rev proteins of HIV-1.

Biochimie - 1 Jan 1991

Karn J, Dingwall C, Finch J T, Heaphy S, Gait M J

Abstract excerpt

HIV-1 tat protein binds specifically to HIV-1 TAR RNA. A Scatchard analysis of tat binding has shown that the purified protein forms a one-to-one complex with HIV-1 TAR RNA with a dissociation constant of Kd = 12 nM. Tat binding in vitro is dependent upon the presence of 3 non-base paired U residues which produce a 'bulge' in the TAR RNA stem-loop structure. Deletion of the uridine residues in the bulge or...

Topics

Base Sequence
Carrier Proteins
Gene Expression Regulation, Viral
Gene Products, rev
Gene Products, tat
Genes, env
HIV Long Terminal Repeat
HIV-1
Microscopy, Electron
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Proviruses

RNA Splicing

RNA, Viral

RNA-Binding Proteins

Transcriptional Activation

rev Gene Products, Human Immunodeficiency Virus

tat Gene Products, Human Immunodeficiency Virus

Source

Open in PubMed

Identifiers

PMID: 1903308
DOI: 10.1016/0300-9084(91)90068-c

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1903308-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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