Article
Mutational analysis of the mitochondrial tRNA genes and flanking regions in umbilical cord tissue from uninfected infants receiving AZT-based therapies for prophylaxis of HIV-1.
Environmental and molecular mutagenesis - 1 Jan 2009
Torres Salina M, Walker Dale M, McCash Consuelo L, Carter Meghan M, Ming Jessica, Cordova Edmund M, Pons Rachel M, Cook Dennis L, Seilkop Steven K, Copeland William C, Walker Vernon E
Abstract excerpt
A sensitive vertical denaturing gradient gel electrophoresis (DGGE) method, using 13 unipolar psoralen-clamped PCR primer pairs, was developed for detecting sequence variants in the 22 tRNA genes and flanking regions (together spanning approximately 21%) of the human mitochondrial genome. A study was conducted to determine (i) if mitochondrial DNA (mtDNA) polymorphisms and/or mutations were detectable in healthy...
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