Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.

Abstract excerpt

Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% identity) to those encoded by the mammalian ERK1/MAP2 kinase and the budding yeast KSS1 and FUS3...

Topics

• Alkaloids
• Amino Acid Sequence
• Animals
• Base Sequence
• Basic-Leucine Zipper Transcription Factors
• Chromosome Mapping
• Cloning, Molecular
• DNA-Binding Proteins
• Drug Resistance, Microbial
• Fungal Proteins