Article
Use of a xylE marker gene to monitor survival of recombinant Pseudomonas putida populations in lake water by culture on nonselective media.
Applied and environmental microbiology - 1 Jul 1991
Winstanley C, Morgan J A, Pickup R W, Saunders J R
Abstract excerpt
IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV1016 and pLV1017, to provide conjugative alternative systems. Detection of the xylE gene carried...
Topics
- Catechol 2,3-Dioxygenase
- Cloning, Molecular
- Culture Media
- Dioxygenases
- Gene Expression
- Genes, Bacterial
- Genetic Markers
- Kinetics
- Mutation
- Oxygenases
- Plasmids
