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Survival of lux-lac-marked biosurfactant-producing Pseudomonas aeruginosa UG2L in soil monitored by nonselective plating and PCR.

Applied and environmental microbiology - 1 May 1994

Flemming C A, Leung K T, Lee H, Trevors J T, Greer C W

Abstract excerpt

Two reporter systems, lacZY and luxAB, were stably integrated into the chromosome of Pseudomonas aeruginosa UG2, a biosurfactant-producing strain. Growth and rhamnolipid production of the UG2 wild-type and reporter gene-bearing UG2L strains were similar in liquid culture. A spontaneous rifampin-resistant detecting UG2Lr, allowed antibiotic selection. Phenotypic characteristics were compared for usefulness in...

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Identifiers

PMID: 8017939
DOI: 10.1128/aem.60.5.1606-1613.1994
PMCID: PMC201524

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