Article
Histone H3 K36 methylation is mediated by a trans-histone methylation pathway involving an interaction between Set2 and histone H4.
Genes & development - 15 Oct 2008
Du Hai-Ning, Fingerman Ian M, Briggs Scott D
Abstract excerpt
Set2-mediated H3 K36 methylation is an important histone modification on chromatin during transcription elongation. Although Set2 associates with the phosphorylated C-terminal domain (CTD) of RNA polymerase II (RNAPII), the mechanism of Set2 binding to chromatin and subsequent exertion of its methyltransferase activity is relatively uncharacterized. We identified a critical lysine residue in histone H4 that is...
Topics
- Antimetabolites
- Blotting, Western
- Chromatin
- Chromatin Immunoprecipitation
- Gene Expression Regulation, Fungal
- Histones
- Immunoblotting
- Immunoprecipitation
- Lysine
- Methylation
- Methyltransferases