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Article

The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.

Molecular cell - 26 Sept 2008

Haruki Hirohito, Nishikawa Junichi, Laemmli Ulrich K

Abstract excerpt

The anchor-away (AA) technique depletes the nucleus of Saccharomyces cerevisiae of a protein of interest (the target) by conditional tethering to an abundant cytoplasmic protein (the anchor) by appropriate gene tagging and rapamycin-dependent heterodimerization. Taking advantage of the massive flow of ribosomal proteins through the nucleus during maturation, a protein of the large subunit was chosen as the...

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Identifiers

PMID: 18922474
DOI: 10.1016/j.molcel.2008.07.020

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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