Article
Characterization of active site structure in CYP121. A cytochrome P450 essential for viability of Mycobacterium tuberculosis H37Rv.
The Journal of biological chemistry - 28 Nov 2008
McLean Kirsty J, Carroll Paul, Lewis D Geraint, Dunford Adrian J, Seward Harriet E, Neeli Rajasekhar, Cheesman Myles R, Marsollier Laurent, Douglas Philip, Smith W Ewen, Rosenkrands Ida, Cole Stewart T, Leys David, Parish Tanya, Munro Andrew W
Abstract excerpt
Mycobacterium tuberculosis (Mtb) cytochrome P450 gene CYP121 is shown to be essential for viability of the bacterium in vitro by gene knock-out with complementation. Production of CYP121 protein in Mtb cells is demonstrated. Minimum inhibitory concentration values for azole drugs against Mtb H37Rv were determined, the rank order of which correlated well with Kd values for their binding to CYP121. Solution-state...
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