Article
High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry.
Nature protocols - 1 Jan 2008
Hong Sun Pyo, Shin Soo-Kyung, Lee Eun Hee, Kim Eun Ok, Ji Seung Il, Chung Hyun Jae, Park Sun Nie, Yoo Wangdon, Folk William R, Kim Soo-Ok
Abstract excerpt
We describe a matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS)-based assay for human papillomavirus (HPV) genotyping--the restriction fragment mass polymorphism (RFMP) assay, which is based on mass measurement of genotype-specific oligonucleotide fragments generated by TypeIIS restriction endonuclease cleavage after recognition sites have been introduced by PCR...
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