Article
High-throughput screening of mouse knockout lines identifies true lean and obese phenotypes.
Obesity (Silver Spring, Md.) - 1 Oct 2008
Brommage Robert, Desai Urvi, Revelli Jean-Pierre, Donoviel Dorit B, Fontenot Gregory K, Dacosta Christopher M, Smith Deon D, Kirkpatrick Laura L, Coker Kenneth J, Donoviel Michael S, Eberhart Derek E, Holt Kathleen H, Kelly Michael R, Paradee William J, Philips Anne V, Platt Kenneth A, Suwanichkul Adisak, Hansen Gwenn M, Sands Arthur T, Zambrowicz Brian P, Powell David R
Abstract excerpt
We developed a high-throughput approach to knockout (KO) and phenotype mouse orthologs of the 5,000 potential drug targets in the human genome. As part of the phenotypic screen, dual-energy X-ray absorptiometry (DXA) technology estimates body-fat stores in eight KO and four wild-type (WT) littermate chow-fed mice from each line. Normalized % body fat (nBF) (mean KO % body fat/mean WT littermate % body fat) values...
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