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Article

Effects of excluded volume upon protein stability in covalently cross-linked proteins with variable linker lengths.

Biochemistry - 19 Aug 2008

Kim Yun Ho, Stites Wesley E

Abstract excerpt

To explore the effects of molecular crowding and excluded volume upon protein stability, we used a series of cross-linking reagents with nine different single-cysteine mutants of staphylococcal nuclease to make covalently linked dimers. These cross-linkers ranged in length from 10.5 to 21.3 A, compelling separations which would normally be found only in the most concentrated protein solutions. The stabilities of...

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Identifiers

PMID: 18656955
DOI: 10.1021/bi800297j
PMCID: PMC2613647

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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