Article
The promoter of the oocyte-specific gene, Gdf9, is active in population of cultured mouse embryonic stem cells with an oocyte-like phenotype.
Methods (San Diego, Calif.) - 1 Jun 2008
Salvador Lisa M, Silva Celso P, Kostetskii Igor, Radice Glenn L, Strauss Jerome F
Abstract excerpt
The study of germ cell-specific gene regulation in vitro is challenging. Here we report that the promoter of the oocyte-specific gene, Gdf9, is active in a population of cultured murine embryonic stem cells (ES) which have a phenotype resembling oocytes. The promoter region of the murine Gdf9 coupled to enhanced green fluorescent protein (eGFP) was stably transfected into XX mouse ES cells. eGFP was expressed...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.