An isotope coding strategy for proteomics involving both amine and carboxyl group labeling.

Abstract excerpt

This paper describes a heavy isotope coding strategy for the analysis of all types of tryptic peptides, including those that are N-terminally blocked and from the C-terminus of proteins. The method exploits differential derivatization of amine and carboxyl groups generated during proteolysis as a means of coding. Carboxyl groups produced during proteolysis incorporate 18O from H218O. Peptides from the C-terminus...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Amines
• Share to Chickens
• Share to Chromatography