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Article

A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.

Nucleic acids research - 25 May 1992

Seyama T, Ito T, Hayashi T, Mizuno T, Nakamura N, Akiyama M

Abstract excerpt

A novel polymerase chain reaction method was developed to preferentially amplify a segment of DNA containing a base substitution mutation. This technique uses a pair of dideoxynucleotide-labeled oligonucleotides (18 mers) of normal sequences as blockers located between the two primers. By virtue of a subtle difference in the melting temperature between the blocker-normal DNA and blocker-mutant DNA hybrids, the...

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Identifiers

PMID: 1598207
DOI: 10.1093/nar/20.10.2493
PMCID: PMC312383

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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