Article
A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.
Nucleic acids research - 25 May 1992
Seyama T, Ito T, Hayashi T, Mizuno T, Nakamura N, Akiyama M
Abstract excerpt
A novel polymerase chain reaction method was developed to preferentially amplify a segment of DNA containing a base substitution mutation. This technique uses a pair of dideoxynucleotide-labeled oligonucleotides (18 mers) of normal sequences as blockers located between the two primers. By virtue...
Topics
- Alleles
- Base Sequence
- DNA, Single-Stranded
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sensitivity and Specificity
- Temperature