Article
High-throughput method for determination of apolipoprotein E genotypes with use of restriction digestion analysis by microplate array diagonal gel electrophoresis.
Clinical chemistry - 1 Nov 1995
Bolla M K, Haddad L, Humphries S E, Winder A F, Day I N
Abstract excerpt
Molecular epidemiological research has identified the association of a common apolipoprotein E (apo E) isoform (E4 as opposed to E3), with risk both of coronary artery disease and of Alzheimer dementia. In addition, the role of apo E genotype (usually E2/E2) in Type III hyperlipidemia is well known. However, both for diagnostic and research purposes, apo E genotyping is cumbersome. The preferred approach is...
Topics
- Apolipoproteins E
- Base Sequence
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Saliva
- Templates, Genetic