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Article

Enhancement of detection and quantification of rotavirus in stool using a modified real-time RT-PCR assay.

Journal of medical virology - 1 Aug 2008

Freeman Molly M, Kerin Tara, Hull Jennifer, McCaustland Karen, Gentsch Jon

Abstract excerpt

A sensitive and specific real-time RT-PCR assay to detect rotavirus in stool samples was optimized and validated using a wide range of rotavirus genotypes. The target of the original TaqMan(R) assay is an 87 bp fragment of the highly conserved non-structural protein 3 (NSP3) gene. Here we modified the original assay by introducing degeneracy into the forward primer to account for sequence variation between...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 18551614
DOI: 10.1002/jmv.21228

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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