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Article

Increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time RT-PCR assay.

Journal of virological methods - 1 Mar 2011

Pang Xiaoli, Cao Min, Zhang Min, Lee Bonita

Abstract excerpt

The real-time TaqMan RT-PCR assay (Pang et al., 2004) did not detect 14 clinical samples with rotavirus G2 genotype. Three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to G2P[4], G2P[8], G3P[4], G9P[4], G8 and G12 sequences. An additional...

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Source

Identifiers

PMID: 21185331
DOI: 10.1016/j.jviromet.2010.12.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-21185331-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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