The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus.

doi:10.1016/j.jviromet.2012.09.021

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus.

Journal of virological methods - 1 Jan 2013

Dung Tran Thi Ngoc, Phat Voong Vinh, Nga Tran Vu Thieu, My Phan Vu Tra, Duy Pham Thanh, Campbell James I, Thuy Cao Thu, Hoang Nguyen Van Minh, Van Minh Pham, Le Phuc Hoang, Tuyet Pham Thi Ngoc, Vinh Ha, Kien Duong Thi Hue, Huy Huynh Le Anh, Vinh Nguyen Thanh, Nga Tran Thi Thu, Hau Nguyen Thi Thu, Chinh Nguyen Tran, Thuong Tang Chi, Tuan Ha Manh, Simmons Cameron, Farrar Jeremy J, Baker Stephen

Abstract excerpt

Rotavirus (RoV) and Norovirus (NoV) are the main causes of viral gastroenteritis. Currently, there is no validated multiplex real-time PCR that can detect and quantify RoV and NoV simultaneously. The aim of the study was to develop, validate, and internally control a multiplex one-step RT real-time PCR to detect and quantify RoV and NoV in stool samples. PCR sensitivity was assessed by comparing amplification...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Caliciviridae Infections

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Diarrhea

Share to Feces

Share to Gastroenteritis

Share to Multiplex Polymerase Chain Reaction

Share to Norovirus

Share to Rotavirus

Share to Rotavirus Infections

Source

Open in PubMed Open full text

Identifiers

PMID: 23046990
DOI: 10.1016/j.jviromet.2012.09.021
PMCID: PMC3528950

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus.PMID 23046990Read ↗

Performance of a one-step quantitative duplex RT-PCR for detection of rotavirus A and noroviruses GII during two periods of high viral circulation.Semantic proximity Read ↗

Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups I and II.Semantic proximity Read ↗

Rapid detection of norovirus genogroup II in clinical and environmental samples using recombinase polymerase amplification.Semantic proximity Read ↗

Detection and characterization of norovirus outbreaks in Germany: application of a one-tube RT-PCR using a fluorogenic real-time detection system.Semantic proximity Read ↗

Evaluation of Various Real-Time Reverse Transcription Quantitative PCR Assays for Norovirus DetectionSemantic proximity Read ↗

A one-step multiplex real-time RT-PCR assay for rapid and simultaneous detection of human norovirus genogroup I, II and IV.Semantic proximity Read ↗

Multiplex real-time RT-PCR for the simultaneous detection and quantification of GI, GII and GIV noroviruses.Semantic proximity Read ↗

Evaluation of RIDA®GENE norovirus GI/GII real time RT-PCR using stool specimens collected from children and adults with acute gastroenteritis.Semantic proximity Read ↗

Use of TaqMan Real-Time Reverse Transcription-PCR for Rapid Detection, Quantification, and Typing of NorovirusSemantic proximity Read ↗

Development of a Recombinase-Aided Amplification Assay for Rapid Detection of Human Norovirus GII.4Semantic proximity Read ↗

Environmental detection of genogroup I, II, and IV noroviruses by using a generic real-time reverse transcription-PCR assay.Semantic proximity Read ↗

Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.