Article
Novel light-upon-extension real-time PCR assay for simultaneous detection, quantification, and genogrouping of group A rotavirus.
Journal of clinical microbiology - 1 May 2010
Nordgren Johan, Bucardo Filemón, Svensson Lennart, Lindgren Per-Eric
Abstract excerpt
We have developed a light-upon-extension (LUX) real-time PCR assay for detection, quantification, and genogrouping of group A rotavirus (RV), the most common cause of acute gastroenteritis in children. The LUX system uses a fluorophore attached to one primer and having a self-quenching hairpin st...
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