Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea.

Journal of medical virology - 1 Mar 2004

Pang Xiaoli L, Lee Bonita, Boroumand Nasim, Leblanc Barbara, Preiksaitis Jutta K, Yu Ip Charlotte C

Abstract excerpt

Six-hundred and twenty-six stool specimens collected from children with diarrhea over a 12-month period were tested for rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay, a conventional nested PCR assay and by electron microscopy (EM). A fragment of 87 bp in a highly-conserved region of non-structural protein 3 (NSP3) in rotavirus genome was amplified by a single-step...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Canada
Share to Diarrhea

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, Viral

Share to Microscopy, Electron

Share to Rotavirus

Share to Rotavirus Infections

Share to Viral Nonstructural Proteins

Source

Identifiers

PMID: 14748075
DOI: 10.1002/jmv.20009

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea.PMID 14748075Read ↗

Enhancement of detection and quantification of rotavirus in stool using a modified real-time RT-PCR assay.Semantic proximity Read ↗

Evaluation and Validation of Real-Time Reverse Transcription-PCR Assay Using the LightCycler System for Detection and Quantitation of NorovirusSemantic proximity Read ↗

Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription-Real-Time Quantitative PCR AssaySemantic proximity Read ↗

Comparison of electron microscopy, ELISA, real time RT-PCR and insulated isothermal RT-PCR for the detection of Rotavirus group A (RVA) in feces of different animal speciesSemantic proximity Read ↗

Detection and Identification of Mammalian Reoviruses in Surface Water by Combined Cell Culture and Reverse Transcription-PCRSemantic proximity Read ↗

Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens.Semantic proximity Read ↗

Standardized positive controls for detection of norovirus by reverse transcription PCRSemantic proximity Read ↗

Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritisSemantic proximity Read ↗

Broadly Reactive Nested Reverse Transcription-PCR Using an Internal RNA Standard Control for Detection of Noroviruses in Stool SamplesSemantic proximity Read ↗

Molecular Detection and Epidemiology of Small Round-Structured Viruses in Outbreaks of Gastroenteritis in the NetherlandsSemantic proximity Read ↗

Detection of Rotaviruses in the Day Care Environment by Reverse Transcriptase Polymerase Chain ReactionSemantic proximity Read ↗

A Multiplex RT-PCR for the Detection of Astrovirus, Rotavirus, and Reovirus in TurkeysSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.