Article
Evaluation of a multiplex real time reverse transcription PCR assay for the detection and quantitation of the most common human rotavirus genotypes.
Journal of virological methods - 1 Mar 2012
Kottaridi Christine, Spathis Aris T, Ntova Chara Kleio, Papaevangelou Vassiliki, Karakitsos Petros
Abstract excerpt
Group A rotaviruses (RVs) are important pathogens that cause acute, dehydrating gastroenteritis in infants and young children. In this study, a multiplex real-time polymerase chain reaction protocol using primers and TaqMan(®) probes specific for viral VP4 and VP7 genes was evaluated. This assay offers simultaneous genotyping and quantification of the most common RV genotypes G1P[8], G2P[4], G3P[8], G4P[8], and...
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