Article
Amino acid residues interacting with both the bound quinone and coenzyme, pyrroloquinoline quinone, in Escherichia coli membrane-bound glucose dehydrogenase.
The Journal of biological chemistry - 8 Aug 2008
Mustafa Golam, Ishikawa Yoshinori, Kobayashi Kazuo, Migita Catharina T, Elias M D, Nakamura Satsuki, Tagawa Seiichi, Yamada Mamoru
Abstract excerpt
The Escherichia coli membrane-bound glucose dehydrogenase (mGDH) as the primary component of the respiratory chain possesses a tightly bound ubiquinone (UQ) flanking pyrroloquinoline quinone (PQQ) as a coenzyme. Several mutants for Asp-354, Asp-466, and Lys-493, located close to PQQ, that were constructed by site-specific mutagenesis were characterized by enzymatic, pulse radiolysis, and EPR analyses. These...
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