Article
An Escherichia coli mutant quinol:fumarate reductase contains an EPR-detectable semiquinone stabilized at the proximal quinone-binding site.
The Journal of biological chemistry - 10 Sept 1999
Hägerhäll C, Magnitsky S, Sled V D, Schröder I, Gunsalus R P, Cecchini G, Ohnishi T
Abstract excerpt
The EPR and thermodynamic properties of semiquinone (SQ) species stabilized by mammalian succinate:quinone reductase (SQR) in situ in the mitochondrial membrane and in the isolated enzyme have been well documented. The equivalent semiquinones in bacterial membranes have not yet been characterized, either in SQR or quinol:fumarate reductase (QFR) in situ. In this work, we describe an EPR-detectable QFR semiquinone...
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