Article
Solution 1H NMR study of the active site structure for the double mutant H64Q/V68F cyanide complex from mouse neuroglobin.
Biophysical chemistry - 1 Aug 2008
Yin Guowei, Li Yanjie, Li Juan, Li Jing, Du Weihong, Wei Qun, Fang Weihai
Abstract excerpt
Solution (1)H NMR spectroscopy has been carried out to investigate the molecular and electronic structures of the active site in H64Q/V68F double mutant mouse neuroglobin in the cyanomet form. Two heme orientations resulting from a 180 degrees rotation about the alpha-gamma-meso axis were observed with a population ratio about 1:1, and the clearly distinguished B isomer was used to perform the study. Based on the...
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