Natural plasmid transformation in Escherichia coli.

doi:10.1007/BF02256071

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Article

Natural plasmid transformation in Escherichia coli.

Journal of biomedical science - 1 Jan 2000

Tsen Suh-Der, Fang Suh-Sen, Chen Mei-Jye, Chien Jun-Yi, Lee Chih-Chun, Tsen Darwin Han-Lin

Abstract excerpt

Although Escherichia coli does not have a natural transformation process, strains of E. coli can incorporate extracellular plasmids into cytoplasm 'naturally' at low frequencies. A standard method was developed in which stationary phase cells were concentrated, mixed with plasmids, and then plated on agar plates with nutrients which allowed cells to grow. Transformed cells could then be selected by harvesting...

Topics

Base Sequence
Calcium
Cell Cycle
DNA, Bacterial
Escherichia coli
Genotype
Magnesium
Phenotype
Plasmids
Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 12065899
DOI: 10.1007/BF02256071

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