Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II.

Abstract excerpt

The high sequence variation of RNA viruses necessitates use of degenerate primers and probes or multiple primers and probes in molecular diagnostic assays. We showed previously that PCR amplification in two rounds, first with long target-specific primers and then with short generic primers, followed by detection using long probes, can tolerate sequence variation. Here we demonstrate that long primers and probes...

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