Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Universal hydrolysis probe-based approach for specific detection and genotyping of foodborne pathogens.

Journal of microbiological methods - 1 Jan 2023

Velez Frank J, Bosilevac Joseph M, Mishra Abhinav, Singh Prashant

Abstract excerpt

Real-time PCR assays are the method of choice for the specific detection of DNA targets. Multiple real-time PCR chemistries are used for developing pathogen detection assays. Among them, a hydrolysis probe is a preferred choice for pathogen detection assays. Two known limitations of hydrolysis probes are high cost and limited storage life. Therefore, this study aimed to develop and validate a universal hydrolysis...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to Escherichia coli Proteins
Share to Food Microbiology

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genotype

Share to Hydrolysis

Share to Shiga-Toxigenic Escherichia coli

Source

Identifiers

PMID: 36460092
DOI: 10.1016/j.mimet.2022.106632

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Universal hydrolysis probe-based approach for specific detection and genotyping of foodborne pathogens.PMID 36460092Read ↗

Hydrolysis probe assays for the detection of pathogenic Enterohemorrhagic Escherichia coli: Multi-Country validation study.Semantic proximity Read ↗

Molecular detection of foodborne pathogens with emphasis on multiplex-allele-specific PCR, computational primer design, and gene amplification approaches.Semantic proximity Read ↗

A Modified Molecular Beacons–Based Multiplex Real-Time PCR Assay for Simultaneous Detection of Eight Foodborne Pathogens in a Single Reaction and Its ApplicationSemantic proximity Read ↗

Development and evaluation of a dual priming oligonucleotide system-based multiplex PCR assay for simultaneous detection of six foodborne pathogensSemantic proximity Read ↗

Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens.Semantic proximity Read ↗

Recent Advances in Molecular Diagnostic Methods for Foodborne Pathogens with a Focus on Listeria monocytogenes and Salmonella sppSemantic proximity Read ↗

Single Laboratory Evaluation of the Q20+ Nanopore Sequencing Kit for Bacterial Outbreak Investigations.Semantic proximity Read ↗

3' Tth Endonuclease Cleavage Polymerase Chain Reaction (3TEC-PCR) Technology for Single-Base-Specific Multiplex Pathogen Detection using a Two-Oligonucleotide System.Semantic proximity Read ↗

Harnessing Non-standard Nucleic Acids for Highly Sensitive Icosaplex (20-Plex) Detection of Microbial Threats for Environmental SurveillanceSemantic proximity Read ↗

A Novel Linear Displacement Isothermal Amplification with Strand Displacement Probes (LDIA-SD) in a Pocket-Size Device for Point-of-Care Testing Of Infectious DiseasesSemantic proximity Read ↗

Integrated Bacterial Identification and Antimicrobial Susceptibility Testing Using PCR and High-Resolution MeltSemantic proximity Read ↗

Development of a recombinase polymerase amplification assay with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella spp. and enteroinvasive Escherichia coliSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.