Article
Short modified antisense oligonucleotides directed against Ha-ras point mutation induce selective cleavage of the mRNA and inhibit T24 cells proliferation.
The EMBO journal - 1 May 1991
Saison-Behmoaras T, Tocqué B, Rey I, Chassignol M, Thuong N T, Hélène C
Abstract excerpt
We have used derivatized antisense oligodeoxynucleotides both in vitro and in vivo specifically to inhibit translation of the activated human oncogene Ha-ras. The oligonucleotides (5'-CCACACCGA-3') were targeted to a region of Ha-ras mRNA including the point mutation G----T at the 12th codon which leads to a Gly----Val substitution in the ras p21 protein. They were linked to an intercalating agent and/or to a...
Topics
- Animals
- Base Sequence
- Cell Transformation, Neoplastic
- Endoribonucleases
- Gene Expression Regulation, Neoplastic
- Genes, ras
- Humans
- Hydrolysis
- Molecular Sequence Data
- Mutation