Article
Deoxynucleotides can replace dideoxynucleotides in minisequencing by arrayed primer extension.
BioTechniques - 1 Mar 2006
Tebbutt Scott J, Mercer Gareth D, Do Ron, Tripp Ben W, Wong Alyson W M, Ruan Jian
Abstract excerpt
Scientific literature describing arrayed primer extension and other array-based minisequencing technologies consistently cite the requirement for four fluorescent dideoxynucleotides (with concomitant absence/inactivation of deoxynucleotides) to ensure single-base extension and thus sequence-specific intensity data that can be interpreted as a base call or genotype. We present compelling evidence that fluorescent...
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