Mechanism of enhanced melibiose transport rate catalyzed by an Escherichia coli lactose carrier mutant with leucine substituted for serine-306. The pH-dependence of melibiose efflux.

Abstract excerpt

The mechanism of melibiose transport was studied in cells containing plasmid pAA22 which expresses the mutant lactose carrier (serine-306 to leucine) cloned from Escherichia coli AA22. These studies were of interest because several lines of evidence suggested that the AA22 mutation conferred novel properties upon the lactose carrier, decreasing turnover with several beta-galactoside substrates, increasing...

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