Article
[Probe of the effect of Asp44 on the stability of myoglobin by circular dichroism spectropolarimeter].
Guang pu xue yu guang pu fen xi = Guang pu - 1 Feb 2008
Wang Jing-Yun, Liu Dan, Tang Qian, Bao Yong-Ming, Zheng Xue-Fang, An Li-Jia
Abstract excerpt
To characterize the roles played by surface-charged residue Asp44 in the structure stability of horse heart myoglobin, the code of Asp44, GAT, in the gene of horse heart myoglobin was changed into AAA for Lys by PCR site-directed mutagenesis. The mutant gene was ligated into PstI/BamHI-cut pGYM and the resulting plasmid was transformed into E. coli BL21. The mutant protein (D44K) was expressed in BL21...
Topics
- Animals
- Aspartic Acid
- Circular Dichroism
- Horses
- Hydrogen-Ion Concentration
- Mutation
- Myoglobin
- Protein Folding
- Protein Stability
- Thermodynamics