Article
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant.
The Biochemical journal - 15 May 2001
Becker D, Braet C, Brumer H, Claeyssens M, Divne C, Fagerström B R, Harris M, Jones T A, Kleywegt G J, Koivula A, Mahdi S, Piens K, Sinnott M L, Ståhlberg J, Teeri T T, Underwood M, Wohlfahrt G
Abstract excerpt
The crystal structures of Family 7 glycohydrolases suggest that a histidine residue near the acid/base catalyst could account for the higher pH optimum of the Humicola insolens endoglucanase Cel7B, than the corresponding Trichoderma reesei enzymes. Modelling studies indicated that introduction of histidine at the homologous position in T. reesei Cel7A (Ala(224)) required additional changes to accommodate the...
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