Article
Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes.
Molecular and cellular biology - 1 Feb 1991
McCusker J H, Yamagishi M, Kolb J M, Nomura M
Abstract excerpt
Starting with two temperature-sensitive mutants (rpa190-1 and rpa190-5) of Saccharomyces cerevisiae, both of which are amino acid substitutions in the putative zinc-binding domain of the largest subunit (A190) of RNA polymerase I, we have isolated many independent pseudorevertants carrying extrag...
Topics
- Binding Sites
- Blotting, Southern
- Chromosome Mapping
- Chromosomes, Fungal
- DNA Transposable Elements
- DNA, Fungal
- Genotype
- Mutation
- RNA Polymerase I
- Restriction Mapping
- Saccharomyces cerevisiae
- Suppression, Genetic
- Temperature
- Zinc