Article
Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I.
Molecular and cellular biology - 1 Feb 1991
Yano R, Nomura M
Abstract excerpt
The SRP3-1 mutation is an allele-specific suppressor of temperature-sensitive mutations in the largest subunit (A190) of RNA polymerase I from Saccharomyces cerevisiae. Two mutations known to be suppressed by SRP3-1 are in the putative zinc-binding domain of A190. We have cloned the SRP3 gene by using its suppressor activity and determined its complete nucleotide sequence. We conclude from the following evidence...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Genes, Fungal
- Genes, Suppressor
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Plasmids