Article
Induction of multiple replacement mutations by oligonucleotide-directed mutagenesis with extended mismatch primers.
Gene analysis techniques - 1 Jan 2000
Lichtler A, Hager G L
Abstract excerpt
We have developed a method called oligo-scanning mutagenesis that uses oligonucleotides to mutate up to 12 contiguous bases in a single step. Some advantages of this procedure are that the position and sequence of the replacement mutations are completely specified by the investigator, and combina...
Topics
- Base Sequence
- Coliphages
- DNA, Viral
- Escherichia coli
- Genetic Engineering
- Methylation
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotides