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Article

Amplification of sequences from affected individuals.

Current protocols in human genetics - 1 May 2001

Watkins H C

Abstract excerpt

This unit describes methods for obtaining DNA sequences from an individual affected by a genetic disorder. Target sequences that may be present at very low copy number in patient samples are amplified by the polymerase chain reaction (PCR). The first Basic Protocol 1 describes harvesting of mRNA from peripheral lymphocytes, which can even be utilized for genes with tissue-specific patterns of expression. This...

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Share to DNA, Complementary
Share to Genetic Diseases, Inborn
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Nucleic Acid Amplification Techniques

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Identifiers

PMID: 18428303
DOI: 10.1002/0471142905.hg0701s00

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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