Article
[Application of double created restriction site PCR-RFLP to identify MGMT gene polymorphisms].
Wei sheng yan jiu = Journal of hygiene research - 1 Jan 2008
Wang Wei, Miao Wenbin, Qiu Yulan, Xia Zhaolin
Abstract excerpt
OBJECTIVE: To develop a proper assay for identifying single nucleotide polymorphisms( SNPs) of the MGMT gene. METHODS: PCR primers were designed by create restriction site (CRS) method, then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted to identify four SNPs in MGMT gene. RESULTS: By PCR, one primer pair yielded target products containing MGMT84 SNP site, and the other...
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