Article
Detection of the Single Nucleotide Polymorphism at Position rs2735940 in the Human Telomerase Reverse Transcriptase Gene by the Introduction of a New Restriction Enzyme Site for the PCR-RFLP Assay.
Annals of clinical and laboratory science - 1 Sept 2017
Wang Sihua, Ding Mingcui, Duan Xiaoran, Wang Tuanwei, Feng Xiaolei, Wang Pengpeng, Yao Wu, Wu Yongjun, Yan Zhen, Feng Feifei, Yu Songcheng, Wang Wei
Abstract excerpt
It has been shown that the single nucleotide polymorphism (SNP) of the rs2735940 site in the human telomerase reverse transcriptase (hTERT) gene is associated with increased cancer risk. The traditional method to detect SNP genotypes is polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). However, there is a limitation to utilizing PCR-RFLP due to a lack of proper restriction enzyme...
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