Article
A new acycloguanosine-specific supermutant of herpes simplex virus type 1 thymidine kinase suitable for PET imaging and suicide gene therapy for potential use in patients treated with pyrimidine-based cytotoxic drugs.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine - 1 May 2008
Likar Yury, Dobrenkov Konstantin, Olszewska Malgorzata, Vider Elena, Shenker Larissa, Cai Shangde, Pillarsetty Nagavarakishore, Hricak Hedvig, Ponomarev Vladimir
Abstract excerpt
UNLABELLED: The herpes simplex virus type 1 thymidine kinase (HSV1-tk) gene is widely used as a suicide gene in combination with ganciclovir (GCV) and as a nuclear imaging reporter gene with an appropriate reporter probe. Wild-type HSV1-tk recognizes a variety of pyrimidine and acycloguanosine nucleoside analogs, including clinically used antiviral drugs. PET of HSV1-tk reporter gene expression will be...
Topics
- Acyclovir
- Animals
- Antiviral Agents
- Cell Line, Tumor
- Cytotoxins
- Ganciclovir
- Gene Expression Regulation, Viral
- Genes, Reporter
- Genetic Therapy