Article
The vacuolar ATPase of Neurospora crassa is indispensable: inactivation of the vma-1 gene by repeat-induced point mutation.
Genetics - 1 May 1996
Ferea T L, Bowman B J
Abstract excerpt
To analyze the phenotype of cells lacking the vacuolar ATPase, we inactivated the vma-1 gene, which encodes the catalytic subunit of the enzyme. Because preliminary experiments suggested the vma-1 gene was essential, we developed a method of simultaneously inactivating the gene and complementing...
Topics
- Base Sequence
- Crosses, Genetic
- DNA Primers
- Endodeoxyribonucleases
- Genes, Fungal
- Genetic Techniques
- Molecular Sequence Data
- Mutagenesis
- Neurospora crassa
- Phenotype
- Plasmids
- Point Mutation