Article
Inactivation of the Neurospora crassa gene encoding the mitochondrial protein import receptor MOM19 by the technique of "sheltered RIP".
Genetics - 1 Jan 1994
Harkness T A, Metzenberg R L, Schneider H, Lill R, Neupert W, Nargang F E
Abstract excerpt
We have used a technique referred to as "sheltered RIP" (repeat induced point mutation) to create mutants of the mom-19 gene of Neurospora crassa, which encodes an import receptor for nuclear encoded mitochondrial precursor proteins. Sheltered RIP permits the isolation of a mutant gene in one nuc...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Blotting, Western
- DNA, Fungal
- Fungal Proteins
- Genes, Fungal
- Genotype
- Kinetics
- Mitochondria
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Neurospora crassa
- Plasmids
- Point Mutation