Article
Development of a single-tube PCR-pyrosequencing method for the simultaneous and rapid detection of four variant alleles of CYP2C9 gene polymorphism.
Journal of clinical pharmacy and therapeutics - 1 Apr 2008
Okada Y, Nakamura K, Adachi A, Watai Y, Horiuchi R, Yamamoto K
Abstract excerpt
BACKGROUND AND OBJECTIVE: CYP2C9 is a polymorphic enzyme that has been reported to metabolize several clinically useful drugs such as warfarin, phenytoin and non-steroidal anti-inflammatory drugs. We designed a rapid single-tube multiplex assay to detect four variant alleles of the CYP2C9 in a single polymerase chain reaction (PCR) and a single pyrosequencing reaction. METHODS: A multiplex PCR was designed to...
Topics
- Adult
- Aged
- Alleles
- Aryl Hydrocarbon Hydroxylases
- Asian People
- Cytochrome P-450 CYP2C9
- Female
- Genotype
- Humans
- Male
- Middle Aged
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sequence Analysis, DNA