Article
[Construction and characterization of a brucella suis S2 strain with a chloramphenicol resistance marker].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Dec 2007
Mao Kai-Rong, Ding Jia-Bo, Cheng Jun-Sheng, Jiang Yu-Wen, Yao Wen-Sheng
Abstract excerpt
Vaccination has not been used widely because of the interference in the discrimination between infected and vaccinated animals in immune-screening procedures. In the present study, chloramphenicol resistance gene (Cm(r)) was cloned into the genomic DNA of brucella suis S2 strain by homologous recombination with knocking out the WbkC gene, and obtained the recombinant rS2-WbkC. Further study confirmed that...
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