Determination of the transition state ensemble for the folding of ubiquitin from a combination of Phi and Psi analyses.

Abstract excerpt

Protein engineering techniques have emerged as powerful tools for characterizing transition states (TSs) for protein folding. Recently, the Psi analysis, in which double-histidine mutations create the possibility of reversible crosslinking in the native state, has been proposed as an additional approach to the well-established Phi analysis. We present here a combination of these two procedures for defining the...

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