Article
Analysis of T cell receptor beta and gamma genes from peripheral blood, regional lymph node and tumor-infiltrating lymphocyte clones from melanoma patients.
Cancer immunology, immunotherapy : CII - 1 Jan 1991
Albertini M R, Nicklas J A, Chastenay B F, Hunter T C, Albertini R J, Clark S S, Hank J A, Sondel P M
Abstract excerpt
A total of 199 T cell clones from two melanoma patients were derived from progenitor T cells from recurrent melanoma, regional lymph nodes (either involved or uninvolved with malignancy) and peripheral blood by inoculating single cells directly into the wells of microtiter plates before in vitro expansion. The surface marker phenotype of most clones was CD4+CD8-, although some were CD4-CD8+. Genomic DNA prepared...
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