Article
Determinants of nucleotide-binding selectivity of malic enzyme.
PloS one - 1 Jan 2011
Hsieh Ju-Yi, Chen Meng-Chun, Hung Hui-Chih
Abstract excerpt
Malic enzymes have high cofactor selectivity. An isoform-specific distribution of residues 314, 346, 347 and 362 implies that they may play key roles in determining the cofactor specificity. Currently, Glu314, Ser346, Lys347 and Lys362 in human c-NADP-ME were changed to the corresponding residues of human m-NAD(P)-ME (Glu, Lys, Tyr and Gln, respectively) or Ascaris suum m-NAD-ME (Ala, Ile, Asp and His,...
Topics
- Amino Acid Sequence
- Humans
- Malate Dehydrogenase
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Structure-Activity Relationship