Role of threonine 190 in modulating the catalytic function of malate dehydrogenase from a thermophile Thermus flavus.

Article

Role of threonine 190 in modulating the catalytic function of malate dehydrogenase from a thermophile Thermus flavus.

The Journal of biological chemistry - 5 Aug 1991

Nishiyama M, Shimada K, Horinouchi S, Beppu T

Abstract excerpt

Random mutagenesis of malate dehydrogenase from a thermophilic bacterium, Thermus flavus AT-62, had revealed that a Thr190----Ile replacement near the essential catalytic residue His187 caused marked modulation of the catalytic properties. For further exploration of a role of the residue at this...

Topics

Base Sequence
Catalysis
Hydrogen-Ion Concentration
Kinetics
Malate Dehydrogenase
Molecular Sequence Data
Mutation
Oxaloacetates
Plasmids
Thermus
Threonine

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