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Article

The role of a conserved histidine residue in a pyruvate-specific Class II aldolase.

FEBS letters - 15 Oct 2008

Wang Weijun, Seah Stephen Y K

Abstract excerpt

Histidine 45 in HpaI was replaced with alanine (H45A) and glutamine (H45Q). In the aldol cleavage reaction, kcat values were lowered by 78- and 2059-fold while Km values were increased by 100- and 42-fold in H45A and H45Q, respectively, compared to the wild-type enzyme. Both mutants displayed higher dissociation constants towards the metal cofactor, pyruvate and the transition state analogue, oxalate. Pyruvate...

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Identifiers

PMID: 18775708
DOI: 10.1016/j.febslet.2008.08.032

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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